-transparent.png){kind=logo}
A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes: (a) from the ribosomal wash and (b) from the postribosomal supernantant. A comparison is made between these preparations with respect to yield and specific activity. eIF-4A and eIF-4D occur mainly in the postribosomal supernatant; eIF-2, eIF-4C and eIF-5 are more evenly divided over both fractions, whereas eIF-1, eIF-3 and eIF-4B are found almost exclusively in the ribosomal wash. No significant difference in specific activity could be detected when factors from both sources were compared, with a possible exception of eIF-4A and eIF-4D.
Related Publications
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
June 1973,
European journal of biochemistry,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
December 1971,
Proceedings of the National Academy of Sciences of the United States of America,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
January 1978,
Proceedings of the National Academy of Sciences of the United States of America,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
May 1979,
Molecular biology reports,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
January 1981,
Current topics in microbiology and immunology,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
February 1993,
The Journal of biological chemistry,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
February 1980,
FEBS letters,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
January 1982,
Current topics in cellular regulation,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
January 1979,
Methods in enzymology,
A Thomas, and
H Goumans, and
H Amesz, and
R Benne, and
H O Voorma
August 1998,
Nature,
