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Fluorescence polarization technique was applied for the assay of angiotensin I (Al) in human plasma. In this assay system, fluorescein labeled Al (F-Al), which retained the original antigenicity, and antibody to Al was allowed to interact in a cuvette in the instruments yielding an increase in the fluorescence polarization (P) value. Non-labeled Al in the sample blocked the binding of F-Al to the antibody resulting lower P value. Log of antigen concentration and P value was found to exhibit reverse linear proportionality between 0.05 ng to 2 ng/ml of antigen (Al) concentration. The present method was compared with standard radioimmunoassay method and the result showed that data were compatible with each other. The calculation of P value is automated and three cavity filter and optics of the instrument gave reliable results. The method is fast (less than 2 min), sensitive (less than 10 picomole/ml) and simple (no separation step before readout of the results).
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
January 1985,
Journal of immunological methods,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
January 1985,
Annales de biologie clinique,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
January 1984,
Therapeutic drug monitoring,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
May 1994,
The Journal of antimicrobial chemotherapy,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
January 1979,
Analytical biochemistry,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
September 2002,
Analytical chemistry,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
January 1997,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
May 1952,
The Biochemical journal,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
January 2006,
Nature protocols,
H Maeda, and
M Nakayama, and
D Iwaoka, and
T Sato
February 1986,
Clinical chemistry,
