Biomaterial Database

Cell cycle synchronization of the mouse S-180 tumor following alternating period of hydroxyurea-blockade and release. 1979

K Tomita

An attempt at synchronization was carried out by intravenous administration of hydroxyurea (HU) into mice bearing the S-180 solid tumor. Following HU blockade for 6.0 hrs equal to (Tc-Ts) period, 65% of all tumor cells were in S-phase. After 2 cycle blockade; HU blockade for 6 hrs, released for 7.5 hrs and reestablished for 6 hrs, 84% of tumor cells were gathered in S-phase with a sharp peak at G1-S junction. Appropriate cycles of HU infusion and release will synchronize significant increments of S-phase or mitotic cells in the S-180 tumor during predictable time intervals.

UI	MeSH Term	Description	Entries
D007399	Interphase	The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).	Interphases
D008297	Male		Males
D008940	Mitotic Index	An expression of the number of mitoses found in a stated number of cells.	Index, Mitotic,Indices, Mitotic,Mitotic Indices
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D004273	DNA, Neoplasm	DNA present in neoplastic tissue.	Neoplasm DNA
D006918	Hydroxyurea	An antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.	Hydroxycarbamid,Hydrea,Oncocarbide
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012510	Sarcoma 180	An experimental sarcoma of mice.	Sarcoma 180, Crocker,Crocker Sarcoma 180
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus