Biomaterial Database

[Stabilization of dextranase from Penicillium funiculosum and Fusarium solani during heating and freeze-drying]. 1979

V I Maksimov, and T I Danilova, and A D Khasipdzheva, and G A Molodova

Freeze-drying of highly purified dextranse from Penicillium funiculosum and Fusarium solani was accompanied by 90% losses of enzyme activity and solubility. Many carbohydrates were tested as stabilizers, e.g. glucose, maltose, lactose, polyglucine, dextranase hydrolyzate of polyglucine as well as mannitol and ammonium sulfate. Polyglucine, its hydrolyzate, and glucose proved most effective stabilizers. The stabilizing effect of polyglucine hydrolyzate of dextranase during its heating and freeze-drying was compared. The effective concentration of the stabilizer during freeze-drying was 10 times lower than during heating.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010407	Penicillium	A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.	Penicilliums
D003910	Dextranase	Enzyme that hydrolyzes DEXTRAN to OLIGOSACCHARIDES.	Endo-Dextranase,Glucanase 447-G,Endo Dextranase,Glucanase 447 G
D005612	Freeze Drying	Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.	Lyophilization,Drying, Freeze,Dryings, Freeze,Freeze Dryings,Lyophilizations
D005670	Fusarium	A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.	Fusariums
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot