-transparent.png){kind=logo}
A dextranase, produced by Penicillium funiculosum, was purified 1,000-fold to yield the enzyme which was demonstrated by gel electrophoresis and electrofocusing to be a homogeneous protein. The purification method included acetone partition, ammonium sulfate fractionation, gel filtration, iron defecation and precipitation, and diethylaminoethyl-cellulose chromatography. The pure enzyme was also obtained by preparative gel electrophoresis. Gel-permeation chromatography indicates a molecular weight of 41,000. An isoelectric pH of 4.6 was established by electrofocusing. A 1-mg amount of the enzyme hydrolyzes a dextran substrate to yield 27,000 isomaltose reducing units in 2 hr.
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
July 1973,
Biotechnology and bioengineering,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
February 1973,
Deutsche zahnarztliche Zeitschrift,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
January 1983,
Prikladnaia biokhimiia i mikrobiologiia,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
December 1974,
Chemical & pharmaceutical bulletin,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
January 1970,
Scandinavian journal of dental research,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
June 1973,
Biochimica et biophysica acta,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
January 1975,
Prikladnaia biokhimiia i mikrobiologiia,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
December 1974,
Chemical & pharmaceutical bulletin,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
January 1974,
Prikladnaia biokhimiia i mikrobiologiia,
L Chaiet, and
A J Kempf, and
R Harman, and
E Kaczka, and
R Weston, and
K Nollstadt, and
F J Wolf
January 1979,
Prikladnaia biokhimiia i mikrobiologiia,
