[Fibrinogen estimation. A simplified gravimetric method (author's transl)]. 1977

H C Ferreira, and J A Escobar, and F S Nakasato

A simplified gravimetric method is proposed for fibrinogen estimation in plasma. Technic: 0.5 ml plasma (citrated) are diluted to up 5 ml with saline. A stick, 5 to 6 cm long, made of a glass capillary tube, or, aluminium foil, with ends bended as a hock, are weighted up to 0.001 g. Introduce one stick previously weighted in diluted plasma. Place two drops of thrombin (10 U/ml), and mix. In seconds, all fibrinogen is transformed in fibrin, deposited around the stick. Remove the stick with the fibrin, and press gently with the fingers, expelling the liquid in excess. Hang the stick with the fibrin, on the rim of a dry centrifuge tube. Centrifuge at 3.000 rpm for 10 minutes (dry centrifugation). Centrifugal forces acting on the fibrin clot expell all liquid particles. Fibrin consist of a very thin layer adhered on the stick. The weight difference (initial and final), represents the solid fibrin. METHODS difference of weight added by 0.1 of the difference (anticoagulant dilution) x 200 to convert in dl. The method is highly reproductible, and takes about 20 minutes to be completed.

UI MeSH Term Description Entries
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D005340 Fibrinogen Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products. Coagulation Factor I,Factor I,Blood Coagulation Factor I,gamma-Fibrinogen,Factor I, Coagulation,gamma Fibrinogen
D013046 Specific Gravity The ratio of the density of a material to the density of some standard material, such as water or air, at a specified temperature. Relative Density,Densities, Relative,Density, Relative,Gravities, Specific,Gravity, Specific,Relative Densities,Specific Gravities

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