[Turbidimetric micromethod for estimation of fibrinogen (author's transl)]. 1981

M Macart, and L Gerbaut, and J F Lampaert

A turbidimetric micromethod for estimation of fibrinogen, both manual and by automatic analysis, is described. It is based on the use of a reagent containing ammonium sulphate, EDTA and glycerol. The blood may be collected either on heparin, EDTA or trisodium citrate. The correlation with the weight methods (r = 0,980), chronometric methods (r = 0,939) and immunonephelemetric methods (r = 0,985) permits its use as a routine method.

UI MeSH Term Description Entries
D008832 Microchemistry The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D009391 Nephelometry and Turbidimetry Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam. Turbidimetry,Nephelometry,Turbidimetry and Nephelometry
D005340 Fibrinogen Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products. Coagulation Factor I,Factor I,Blood Coagulation Factor I,gamma-Fibrinogen,Factor I, Coagulation,gamma Fibrinogen
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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