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A micro-agglutination test method for detecting antibodies to Brucella canis was developed. Heat-killed Brucella canis antigens were diluted to an optical density of 0.8 at 420 nm using a spectrophotometer. A volume of 0.025 ml of the antigen was incubated with the same volume of serially diluted sera for 18 to 24 hr at 37 C. Titers of selected dog sera obtained by the present micro-test method were well correlated with those obtained by the classical tube test with satisfactory reproducibility. The micro-test method is more advantageous for screening the antibodies of dog sera because the test can be performed with: (1) smaller volume of the test sera and the antigen (2) shorter period for incubation, and (3) lesser labor.
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
March 1973,
Applied microbiology,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
January 1975,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
February 1972,
Applied microbiology,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
June 1977,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
February 1970,
The Journal of infectious diseases,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
June 1982,
American journal of clinical pathology,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
November 1977,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
February 1979,
Journal of bacteriology,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
January 1979,
Avian diseases,
T Serikawa, and
T Muraguchi, and
N Nakao, and
J Yamada
January 1981,
Sangre,
