Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2. 1978

K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson

Complementary strand-specific adenovirus DNA of full length or from endonuclease BamHI fragments was used as a probe to estimate the fractional representation and abundance of viral sequences in five hamster cell lines (Ad2HE1-5) transformed with UV-inactivated adenovirus type 2. The fraction of the viral genome present in the five transformed cell lines varied from 44% in the Ad2HE5 cell line to 84% in the Ad2HE3 cell line. The number of viral DNA copies per diploid cell equivalent ranged from 1.8 in the Ad2HE1 line to 7.1 in the Ad2HE4 line. In vivo labeling with [35S]methionine followed by immunoprecipitation with an antiserum against adenovirus type 2 early proteins revealed virus-specific polypeptides with molecular weights of 42,000 to 58,000 in extracts from all five hamster cell lines. Several other early viral polypeptides were detected in some of the adenovirus type 2-transformed hamster cell lines.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002471 Cell Transformation, Neoplastic Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill. Neoplastic Transformation, Cell,Neoplastic Cell Transformation,Transformation, Neoplastic Cell,Tumorigenic Transformation,Cell Neoplastic Transformation,Cell Neoplastic Transformations,Cell Transformations, Neoplastic,Neoplastic Cell Transformations,Neoplastic Transformations, Cell,Transformation, Cell Neoplastic,Transformation, Tumorigenic,Transformations, Cell Neoplastic,Transformations, Neoplastic Cell,Transformations, Tumorigenic,Tumorigenic Transformations
D002472 Cell Transformation, Viral An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus. Transformation, Viral Cell,Viral Cell Transformation,Cell Transformations, Viral,Transformations, Viral Cell,Viral Cell Transformations
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D000260 Adenoviruses, Human Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G. APC Viruses,APC Virus,Adenovirus, Human,Human Adenovirus,Human Adenoviruses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
March 1982, Journal of virology,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
January 1977, Cell,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
January 1975, Cold Spring Harbor symposia on quantitative biology,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
January 1980, Cold Spring Harbor symposia on quantitative biology,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
April 1981, Journal of molecular biology,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
January 1980, Cold Spring Harbor symposia on quantitative biology,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
February 1980, Proceedings of the National Academy of Sciences of the United States of America,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
November 1976, Journal of virology,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
November 1980, Proceedings of the Royal Society of London. Series B, Biological sciences,
K Johansson, and H Persson, and A M Lewis, and U Pettersson, and C Tibbetts, and L Philipson
July 1978, Cell,
Copied contents to your clipboard!