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New techniques for the adrenalectomy of pigs and for the isolation of pig uterus nuclei are described. The isolated nuclei were analysed for their content of estradiol and of estradiol receptor. The concentration of the latter exceeded that of the hormone in extracts of uterine nuclei from ovariectomized pigs by ratios of 1.8--10.3. Substantial amounts of both monomer and "activated" dimer receptor but no estradiol at all were extracted from uterine nuclei of ovariectomized/adrenalectomized pigs. The mechanism of action of steroid hormones is discussed on the basis of these results.
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