Biomaterial Database

[Attempts at freezing bull semen in a yolk-raffinose medium without glycerin]. 1978

T Stoianov, and B Kostadinov

A new medium was produced, containing no glycerin but rich in raffinose, egg yolk, and mineral water, adequate for freezing bull semen. It was tested parallel to a control extender, consisting of yolk, lactose, and glycerine. The absolute survival rate of the spermatozoa kept at 0-4 degrees C in a raffinose medium proved by 24.25 h higher than that of the spermatozoa in the control medium. No other essential differences were established as to motility, heat resistance and percent of pathologic forms of freshly diluted and frozen semen between the test and the control medium. The biologic test on a total of 133 cows resulted in a 71.4% conception rate at first insemination. The semen frozen in the control medium showed 62.6% conception at the insemination of 123 cows. The difference of 8.8% was in favour of the new synthetic medium. The insufficient number of animals (133) used in the investigations made it necessary to test the new medium on a wider production basis.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D011887	Raffinose	A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.	Gossypose,Melitose,Melitriose
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002452	Cell Count	The number of CELLS of a specific kind, usually measured per unit volume or area of sample.	Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004530	Egg Yolk	Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins.	Egg Yolks,Yolk, Egg,Yolks, Egg
D005260	Female		Females
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D005990	Glycerol	A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, or sweetening agent.	1,2,3-Propanetriol,Glycerin,1,2,3-Trihydroxypropane,Glycerine
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot