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1. Glucuronide formation of bilirubin and p-nitrophenol in vitro with excess of UDP-glucuronic acid by UDP-glucuronyltransferase from livers of young and adult rabbits was studied. 2. The development of UDP-glucuronyltransferase for the two substrates followed a markedly different pattern during maturation of young rabbits, p-nitrophenol-conjugation ability being much higher at birth than that for bilirubin. 3. Mg(2+) increased bilirubin conjugation, but inhibited p-nitrophenyl glucuronide formation. 4. p-Nitrophenol acted as a potent non-competitive inhibitor for bilirubin conjugation but bilirubin did not affect p-nitrophenyl glucuronidation. 5. The enzyme for bilirubin conjugation was inactivated at pH9 during treatment with snake venom, whereas in the same preparation the activity of the corresponding enzyme for p-nitrophenol was enhanced. In addition, some solubilization of the latter enzyme could be achieved by this method. 6. The possibility of the existence of more than one enzyme system for the formation of O-glucuronides is discussed.
G A Tomlinson, and
S J Yaffe
November 1972,
Biochimica et biophysica acta,
G A Tomlinson, and
S J Yaffe
October 1971,
Biochemical and biophysical research communications,
G A Tomlinson, and
S J Yaffe
December 1981,
FEBS letters,
G A Tomlinson, and
S J Yaffe
May 1973,
Biochemical pharmacology,
G A Tomlinson, and
S J Yaffe
December 1952,
Acta physiologica latino americana,
G A Tomlinson, and
S J Yaffe
April 1978,
Xenobiotica; the fate of foreign compounds in biological systems,
G A Tomlinson, and
S J Yaffe
January 1966,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
G A Tomlinson, and
S J Yaffe
June 1977,
Clinical chemistry,
G A Tomlinson, and
S J Yaffe
June 1991,
Gastroenterologia Japonica,
G A Tomlinson, and
S J Yaffe
April 1981,
The Journal of clinical investigation,
