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The pool sizes of the choline intermediates and cofactors involved in the CDPcholine pathway were studied in alveolar type II cells from adult rat lung. The choline phosphate pool was much larger than both the choline and CDPcholine pools. Kinetic analysis of the pool sizes revealed that cholinephosphate cytidylyltransferase catalyzes a non-equilibrium reaction. These results are consistent with the idea that cholinephosphate cytidylyltransferase regulates the choline flux into phosphatidylcholine in adult rat lung type II cells.
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