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A T-lymphotropic retrovirus with cytopathic but not immortalizing activity has been isolated repeatedly from patients with acquired immune deficiency (AIDS) or lymphadenopathy syndrome (LAS) and successfully transmitted to a T-cell line (HT) for continuous production. Seroepidemiology data and the OKT4 tropism and cytopathogenicity of this virus indicate it is the etiological agent of AIDS. We have cloned HTLV-III genomes using three approaches: (1) cDNA clones were obtained from a cDNA plasmid library constructed from RNA of purified virions using oligo (dT) primers; (2) unintegrated provirus clones were obtained from Hirt supernatants of acutely infected H9 cells using virus from H9/HTLV-III; (3) clones of integrated provirus with flanking cellular sequences were obtained from a genomic DNA library of H9/HTLV-III. Analyses of these clones show that the HTLV-III genome is similar in size to those of HTLV-I and HTLV-II and contains a gene that functions as a transcriptional activator. Different isolates of HTLV-III display greater polymorphism than different isolates of HTLV-I among each other, possibly due to the highly replicative nature of HTLV-III. Viral sequences could be detected in fresh lymph node tissues of some AIDS patients, but even in the positive samples the number of infected cells is small (less than 1%). In both fresh tissues that are positive for viral sequences and HTLV-III infected cell lines, a substantial amount of unintegrated viral DNA is present in addition to integrated provirus. This is an unusual finding for retroviruses but may be significant in the cytopathicity of HTLV-III as has been proposed for some avain retroviruses.
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
May 1992,
Human pathology,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
November 1985,
Annals of internal medicine,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
April 1986,
Journal of virology,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
June 1984,
Lancet (London, England),
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
September 1985,
Blood,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
September 1985,
Lancet (London, England),
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
December 1985,
MMWR. Morbidity and mortality weekly report,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
August 1985,
The Journal of clinical investigation,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
September 1985,
JAMA,
F Wong-Staal, and
B H Hahn, and
G M Shaw, and
S K Arya, and
M Harper, and
M Gonda, and
R Gilden, and
L Ratner, and
B Starcich, and
T Okamoto
January 1985,
Pediatric infectious disease,
