-transparent.png){kind=logo}
The present study defines criteria for determining the presence of estrogen-receptors in human breast carcinomas demonstrated by a histochemical assay using 17 beta-estradiol-carboxy-methyl-oxim-bovine serum albumen-FITC. The criteria were: 1) the percentage of cells showing fluorescence; 2) the intensity of the fluorescence observed, and 3) the percentage of epithelial structures in tissue specimens. Using these predefined criteria in 132 human breast carcinomas as 91.6% agreement was found between the results of the histochemical assay and those of the biochemical Charcoal method. The main causes of disagreement (7 of the 11 cases) were sampling errors between the tissue specimens used for the histochemical and biochemical assay, and an insufficient percentage of epithelial structures (less than 15%) to allow biochemical identification of estrogen receptor activity. In the hands of pathologists with experience of the field of histochemistry this histochemical assay may be the method of choice for the assessment of estrogen receptors.
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
May 1982,
Cancer,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
December 1980,
Cancer,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
May 1979,
American journal of clinical pathology,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
February 1981,
Gan,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
June 1999,
Asian Pacific journal of allergy and immunology,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
May 1987,
Cancer research,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
May 1983,
Gan no rinsho. Japan journal of cancer clinics,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
January 1984,
Virchows Archiv. A, Pathological anatomy and histopathology,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
March 1996,
Revista medica de Chile,
C J Meijer, and
J van Marle, and
J P Persijn, and
W van Niewenhuizen, and
J P Baak, and
M E Boon, and
J Lindeman
January 1986,
Applied pathology,
