Biomaterial Database

Serotypes of red clover necrotic mosaic virus. II. Typing of 34 isolates. 1982

M Musil, and O Lesková, and J Gallo

Examination of 34 isolates of red clover necrotic mosaic virus (RCNMV) from Czechoslovakia in agar gel double diffusion precipitation tests and immunoelectrophoresis in agarose gel showed that 16 isolates belonged to serotype B, 6 isolates to serotype C and 4 isolates to serotype A; 3, 1 and 4 isolates represented mixtures of serotypes A + B, A + C and B + C, respectively. The distribution of the individual RCNMV serotypes in Czechoslovakia is not bound to definite geographic areas; two serotypes were even involved in mixed infections of single plants. Isolates of a given serotype were serologically and electrophoretically identical with the respective type isolate, i.e. there was a correlation between antigenic properties and electrophoretic mobility.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D009029	Mosaic Viruses	Viruses which produce a mottled appearance of the leaves of plants.	Mosaic Virus,Virus, Mosaic,Viruses, Mosaic
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings