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Transport properties of 9-(S)-(2,3-dihydroxypropyl) adenine (DHPA) in cell cultures were studied. Transport of DHPA into chick embryo (CE), ZP (a cell line derived from rabbit lungs) and HeLa cells reached equilibrium values after 10 min incubation. The concentration of intracellular DHPA varied from 30 to 50 per cent of that in the medium. DHPA transport was only slightly affected during the lag phase of vaccinia virus replication. The opinion that DHPA is transported into the cell by facilitated diffusion, is supported 1) by the data on DHPA transport as a function of temperature and extracellular concentration, 2) by evidence of countertransport, 3) by temperature-dependent exit of DHPA, 4) by specific inhibition of DHPA transport in the presence of adenosine and deoxyadenosine and 5) by the fact that intracellular concentration of DHPA in equilibrium does not reach the concentration of DHPA in the medium. V and KM values varied in the range of 2-17 pmoles/min per 10(6) cells and 4-7 microM, respectively.
M Dragún, and
B Rada, and
A Holý
October 1984,
Bioorganicheskaia khimiia,
M Dragún, and
B Rada, and
A Holý
August 1981,
Biochemical pharmacology,
M Dragún, and
B Rada, and
A Holý
December 1981,
Teratology,
M Dragún, and
B Rada, and
A Holý
May 1983,
Acta virologica,
M Dragún, and
B Rada, and
A Holý
July 1981,
Toxicology and applied pharmacology,
M Dragún, and
B Rada, and
A Holý
May 1978,
Science (New York, N.Y.),
M Dragún, and
B Rada, and
A Holý
May 1978,
Science (New York, N.Y.),
M Dragún, and
B Rada, and
A Holý
December 1980,
Nucleic acids research,
M Dragún, and
B Rada, and
A Holý
January 1980,
Chemotherapy,
M Dragún, and
B Rada, and
A Holý
September 1980,
Acta virologica,
