Biomaterial Database

A new sensitive, thiol tolerant method for the determination of inorganic pyrophosphatase. 1983

R Lahti, and I Jantunen, and J Heinonen

In this work a new sensitive, thiol tolerant method for the determination of inorganic pyrophosphatase (EC 3.6.1.1) was developed. It is based on the formation of phosphomolybdate-triethylamine complex, which in acid-acetone mixture forms a white, homogeneous precipitate, the turbidity of which can accurately measured spectrophotometrically at 355 nm. This method can be easily applied to all the Pi-producing enzymes whose substrates are stable enough in our acidic experimental conditions.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D011755	Pyrophosphatases	A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.	Pyrophosphatase
D004355	Drug Stability	The chemical and physical integrity of a pharmaceutical product.	Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry
D013293	Enterococcus faecalis	A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.	Streptococcus Group D,Streptococcus faecalis
D013438	Sulfhydryl Compounds	Compounds containing the -SH radical.	Mercaptan,Mercapto Compounds,Sulfhydryl Compound,Thiol,Thiols,Mercaptans,Compound, Sulfhydryl,Compounds, Mercapto,Compounds, Sulfhydryl
D043564	Inorganic Pyrophosphatase	An enzyme which catalyzes the hydrolysis of diphosphate (DIPHOSPHATES) into inorganic phosphate. The hydrolysis of pyrophosphate is coupled to the transport of HYDROGEN IONS across a membrane.	Pyrophosphatase, Inorganic,H(+)-PPase,H+-Pyrophosphatase,Proton-Pumping Inorganic Pyrophosphatase,Proton-Translocating Pyrophosphatase,Pyrophosphate-Energized Inorganic Pyrophosphatase,H+ Pyrophosphatase,Inorganic Pyrophosphatase, Proton-Pumping,Inorganic Pyrophosphatase, Pyrophosphate-Energized,Proton Pumping Inorganic Pyrophosphatase,Proton Translocating Pyrophosphatase,Pyrophosphatase, Proton-Pumping Inorganic,Pyrophosphatase, Proton-Translocating,Pyrophosphatase, Pyrophosphate-Energized Inorganic,Pyrophosphate Energized Inorganic Pyrophosphatase