BIOMAT Database Logo BIOMAT Database Logo

Determination of 5-hydroxyindoleacetic acid in urine by high-performance liquid chromatography: a fully automated method. 1981

J P Garnier, and B Bousquet, and C Dreux

High-performance liquid anion-exchange chromatography is combined with continuous flow spectrofluorometry at an optimized pH for the determination of 5-hydroxyindoleacetic acid (5-HIAA) in urine. Its chief advantages are speed (7 min per determination), enabling measurement of large series of samples, precision (coefficient of variation = 0.72%), sensitivity (detection limit: 1 pmol) and specificity (no interference with 5-HIAA precursors or with the drugs tested). Reference values (n = 112) are 25 +/- 25 mumol per 24 h. Comparison with a reference spectrofluorometric technique shows a correlation coefficient of 0.96.

UI MeSH Term Description Entries
D007414 Intestinal Neoplasms Tumors or cancer of the INTESTINES. Cancer of Intestines,Intestinal Cancer,Cancer of the Intestines,Intestines Cancer,Intestines Neoplasms,Neoplasms, Intestinal,Cancer, Intestinal,Cancer, Intestines,Cancers, Intestinal,Cancers, Intestines,Intestinal Cancers,Intestinal Neoplasm,Intestines Cancers,Intestines Neoplasm,Neoplasm, Intestinal,Neoplasm, Intestines,Neoplasms, Intestines
D007421 Intestine, Small The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM. Small Intestine,Intestines, Small,Small Intestines
D002276 Carcinoid Tumor A usually small, slow-growing neoplasm composed of islands of rounded, oxyphilic, or spindle-shaped cells of medium size, with moderately small vesicular nuclei, and covered by intact mucosa with a yellow cut surface. The tumor can occur anywhere in the gastrointestinal tract (and in the lungs and other sites); approximately 90% arise in the appendix. It is now established that these tumors are of neuroendocrine origin and derive from a primitive stem cell. (From Stedman, 25th ed & Holland et al., Cancer Medicine, 3d ed, p1182) Argentaffinoma,Carcinoid,Carcinoid, Goblet Cell,Argentaffinomas,Carcinoid Tumors,Carcinoids,Carcinoids, Goblet Cell,Goblet Cell Carcinoid,Goblet Cell Carcinoids,Tumor, Carcinoid,Tumors, Carcinoid
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006897 Hydroxyindoleacetic Acid 5-HIAA,5-Hydroxy-3-Indoleacetic Acid,5-Hydroxyindolamine Acetic Acid,5 Hydroxy 3 Indoleacetic Acid,5 Hydroxyindolamine Acetic Acid,Acetic Acid, 5-Hydroxyindolamine,Acid, 5-Hydroxy-3-Indoleacetic,Acid, 5-Hydroxyindolamine Acetic,Acid, Hydroxyindoleacetic
D001331 Automation Controlled operation of an apparatus, process, or system by mechanical or electronic devices that take the place of human organs of observation, effort, and decision. (From Webster's Collegiate Dictionary, 1993) Automations

Related Publications

J P Garnier, and B Bousquet, and C Dreux
Automated liquid chromatographic determination of 5-hydroxyindoleacetic acid in urine.
February 1971, Clinical chemistry,
J P Garnier, and B Bousquet, and C Dreux
Quantitative determination of suprofen in human plasma and urine by fully automated high-performance liquid chromatography.
January 1982, Arzneimittel-Forschung,
J P Garnier, and B Bousquet, and C Dreux
Determination of vanillylmandelic, 5-hydroxyindoleacetic and homovanillic acid in urine by isocratic liquid chromatography.
January 1997, European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
J P Garnier, and B Bousquet, and C Dreux
Two-dimensional high-performance liquid chromatographic determination of 5-hydroxyindoleacetic acid and homovanillic acid in urine.
January 1985, Analytical biochemistry,
J P Garnier, and B Bousquet, and C Dreux
Analysis of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid in human urine by high-performance liquid chromatography and fluorometry.
October 1984, Journal of chromatography,
J P Garnier, and B Bousquet, and C Dreux
Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra-performance Liquid Chromatography Tandem Mass Spectrometry.
January 2022, Methods in molecular biology (Clifton, N.J.),
J P Garnier, and B Bousquet, and C Dreux
[Determination of phenylglyoxylic acid and mandelic acid in urine by high performance liquid chromatography method].
October 2017, Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,
J P Garnier, and B Bousquet, and C Dreux
[Determination of vanillymandelic acid in urine by high performance liquid chromatography].
October 2000, Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University,
J P Garnier, and B Bousquet, and C Dreux
[Determination of oxalic acid in urine by high-performance liquid chromatography].
March 1986, Revista espanola de fisiologia,
J P Garnier, and B Bousquet, and C Dreux
Automated method for urine 5-hydroxyindole-acetic acid by high-performance liquid chromatography using Gilson ASTED sample preparation unit.
January 2001, Annals of clinical biochemistry,
Copied contents to your clipboard!