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The nature of the stimulation by albumin of amylase activity determined by use of blue starch polymer was studied. The binding of albumin to the starch polymer was responsible for the stimulation of amylase activity, since the stimulation was observed with the albumin-bound blue starch polymer as substrate irrespective of the presence or absence of free albumin in the reaction mixture. When the albumin-bound blue starch polymer was hydrolysed by amylase, the blue oligosaccharide fragments were released with the albumin attached. These oligosaccharide fragments were about 1.3 times larger in average molecular size than the fragments released in the absence of albumin, suggesting that the apparent stimulation of amylase by albumin, (about 1.3 times) is due to the liberation of the larger oligosaccharide fragments.
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
August 1971,
Rinsho byori. The Japanese journal of clinical pathology,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
August 1977,
Rinsho byori. The Japanese journal of clinical pathology,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
January 1977,
Acta Universitatis Carolinae. Medica. Monographia,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
May 1970,
American journal of clinical pathology,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
November 1972,
Clinica chimica acta; international journal of clinical chemistry,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
February 1973,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
May 1997,
Analytical biochemistry,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
October 1971,
Clinical biochemistry,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
March 1975,
Scandinavian journal of clinical and laboratory investigation,
H Akedo, and
K Yoshioka, and
M Ehara, and
T Kitamura
January 1973,
Biokhimiia (Moscow, Russia),
