-transparent.png){kind=logo}
Further characterization of three polypeptide antigens present in Novikoff ascites hepatoma (molecular weights 39,000, 49,000, and 56,000--p39, p49, and p56, respectively), has established the identity of these proteins as cytokeratins. Two-dimensional gel electrophoresis revealed that each of the three proteins exists in several isoelectric forms, all characteristic of the keratins. The antigens p39, and p56 had amino acid compositions compatible with this family of proteins, and immunofluorescence microscopy localized the antigens on elaborate filament arrays indicative of the cytokeratins. Antibodies monospecific for the p39 antigen, purified by using affinity chromatography, were used to demonstrate that protein p39 was specific for Novikoff hepatoma cells. These results suggest that the cytokeratins contain cell-specific that can be useful as markers for differentiation and neoplasia.
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
July 1985,
Biochemistry,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
May 1980,
Cancer research,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
July 1978,
Cancer research,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
April 1965,
The Biochemical journal,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
February 1967,
The Biochemical journal,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
April 1982,
Cancer research,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
April 1978,
Proceedings of the National Academy of Sciences of the United States of America,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
February 1973,
The Journal of biological chemistry,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
November 1969,
The Biochemical journal,
W N Schmidt, and
R L Pardue, and
M C Tutt, and
R C Briggs, and
B R Brinkley, and
L S Hnilica
December 1984,
Cancer research,
