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Three stable monoclonal antibody-producing mouse hybridoma lines have been developed which produce high-titer, immunoglobulin M antibodies specific for the Novikoff ascites hepatoma (NAH) Mr 39,000 cytokeratin antigen (p39). Immunotransfer assays of cytoskeletal protein-enriched fractions indicated p39 to be present in a range of rat tissues, including colon, breast, lung, and uterus. Two-dimensional gel immunoblots confirmed that immunoreactivity in the latter tissues was for polypeptides with similar isoelectric points to those of NAH p39; however, reactivity in the colon contained a wide range of additional isomeric forms. Immunohistochemical localization studies with these antibodies revealed enrichment of p39 in the simple epithelia or ductular structures of organs containing this antigen. In all sections examined, glandular epithelia were observed to be only weakly immunoreactive. Additional immunoblot and immunocytochemical analyses with the monoclonal antibodies and polyspecific antisera to NAH cytokeratin suggest the human Mr 40,000 cytokeratin to be similar to but not identical to NAH p39.
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
January 1984,
Proceedings of the National Academy of Sciences of the United States of America,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
July 1985,
Biochemistry,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
May 1982,
Proceedings of the National Academy of Sciences of the United States of America,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
April 1982,
Cancer research,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
August 1981,
The Journal of biological chemistry,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
January 1979,
Cancer research,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
April 1986,
Molecular and cellular biochemistry,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
April 1975,
Cancer research,
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
January 1985,
Hepatology (Baltimore, Md.),
W N Schmidt, and
D L Page, and
C A Schmidt, and
K B McKusick, and
W S Ward, and
L S Hnilica
February 1988,
Molecular and cellular biochemistry,
