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Hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse albumin and alpha-fetoprotein synthesis. Karyotype analyses suggest that all parental chromosomes are present in the hybrids. The extinction, therefore, of mouse hepatocyte genes is attributed to the inhibitory action of the rat genome. In these studies, we show that these hybrids possess and express the mouse beta-glucuronidase gene (which is encoded on the same chromosome as the mouse albumin and alpha-fetoprotein gene), and we present data of Southern blot analysis which demonstrate that such hybrids have indeed retained both mouse and rat albumin DNA sequences. In addition, using mouse albumin cDNA, we have shown by cDNA-RNA reassociation kinetics that albumin mRNA is virtually absent in these hybrids. We conclude from these studies that the extinction of albumin synthesis involves a mechanism which results in the loss of cytoplasmic albumin mRNA.
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
September 1984,
Somatic cell and molecular genetics,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
March 1972,
Proceedings of the National Academy of Sciences of the United States of America,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
January 1975,
Somatic cell genetics,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
April 1996,
Biochemical genetics,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
January 1983,
Isozymes,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
July 1975,
Somatic cell genetics,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
January 1984,
Differentiation; research in biological diversity,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
January 1991,
Somatic cell and molecular genetics,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
July 1976,
Somatic cell genetics,
J Papaconstantinou, and
E Wong, and
H Ratrie, and
C Szpirer, and
J Szpirer
March 1974,
Proceedings of the National Academy of Sciences of the United States of America,
