BIOMAT Database Logo BIOMAT Database Logo

[Physico-chemical properties of several phages of Bacillus thuringiensis]. 1977

V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian

A study was made of biological and physico-chemical properties of phages of Bac. thuringiensis as well as of a number of parameters of nucleic acids isolated from these phages. The phages contain double-stranded DNA. Molecular weights of DNA from three phages--Tg9, Tg10 and Tg13 have been determined by two independent methods: by measuring the contour length of DNA, from the sedimentation constant and for DNA of phage Tg10 also by endonuclease EcRI hydrolysis. These methods gave similar results. On the basis of the temperature of DNA melting the content of GC pairs was found equal to 37.9, 33.4 and 35.1 mole% for DNA's of phage Tg9, Tg10 and Tg13, respectively. On the basis of measuring the intervals of DNA melting a conclusion was made that DNA of the Tg9 and Tg13 phage has a random distribution of base pairs, while DNA of phage Tg10 displays some clustering of base distribution along the molecule. It has been shown that restrictase EcoRI hydrolyses phage Tg10 DNA into 6 fragments of different molecular weights; DNA's of Tg9 and Tg13 phages are not hydrolyzed. A possibility of existance of phage Tg10 DNA in linear and ring forms has been established. The characteristics of phage particles have been determined by electron microscopy.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009691 Nucleic Acid Denaturation Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible. DNA Denaturation,DNA Melting,RNA Denaturation,Acid Denaturation, Nucleic,Denaturation, DNA,Denaturation, Nucleic Acid,Denaturation, RNA,Nucleic Acid Denaturations
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004270 DNA, Circular Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992) Circular DNA,Circular DNAs,DNAs, Circular
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D001413 Bacillus thuringiensis A species of gram-positive bacteria which may be pathogenic for certain insects. It is used for the biological control of the Gypsy moth. Bacilan,Dipel,Thuricide
D001435 Bacteriophages Viruses whose hosts are bacterial cells. Phages,Bacteriophage,Phage
D014466 Ultraviolet Rays That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants. Actinic Rays,Black Light, Ultraviolet,UV Light,UV Radiation,Ultra-Violet Rays,Ultraviolet Light,Ultraviolet Radiation,Actinic Ray,Light, UV,Light, Ultraviolet,Radiation, UV,Radiation, Ultraviolet,Ray, Actinic,Ray, Ultra-Violet,Ray, Ultraviolet,Ultra Violet Rays,Ultra-Violet Ray,Ultraviolet Black Light,Ultraviolet Black Lights,Ultraviolet Radiations,Ultraviolet Ray

Related Publications

V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Physico-chemical properties of Bacillus thuringiensis IMV B-7324 fibrinolytic peptidase].
January 2013, Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993),
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Physico-chemical properties of there phages of Pseudomonas syringae].
January 1997, Ukrainskii biokhimicheskii zhurnal (1978),
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Comparative study of the physicochemical and biological properties of Bacillus thuringiensis phages].
January 1986, Mikrobiologiia,
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Structural characteristics of DNA of Bacillus thuringiensis phages].
January 1986, Mikrobiologiia,
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Several physico-chemical properties of manganese ore and quartz dust].
July 1970, Gigiena i sanitariia,
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Chemical and physico-chemical properties of the exo-enterotoxin of Bacillus cereus].
January 1973, Biokhimiia (Moscow, Russia),
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Biology of two lysogenic phages from Bacillus thuringiensis MZ1].
February 2007, Wei sheng wu xue bao = Acta microbiologica Sinica,
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
[Purification and physico-chemical properties of proteolytic complex Bacillus sp].
January 2009, Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993),
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
Structure of two phages of Bacillus thuringiensis and B. cereus.
January 1974, Canadian journal of microbiology,
V G Bogush, and V B Smirnov, and B A Rebentish, and V I Permogorov, and R R Azizbekian
Occurrence of Bacillus thuringiensis and their phages in Yemen soil.
January 2014, Virusdisease,
Copied contents to your clipboard!