[Physico-chemical properties of there phages of Pseudomonas syringae]. 1997

A L Boĭko, and L I Semchuk, and L V Tokarchuk, and S A Romashev
Taras Shevchenko National University, Kyiv, Ukraine.

The properties of DNA for 9B, 123, 788/8 phages lysing phytopathogenic Pseudomonas syringae bacteria have been analysed with results presented. It was ascertained that their genomes consist of GC-type two-chain DNA molecules having molecular weight 14-15 mDa. It was shown that sedimentation coefficient for all three phage DNA is identical and equals 26S. GC-base percentage was calculated for the phage genomes. Its value, according to results of sedimentation analysis and melting temperature, was the same for 9B (51%, 57%) and 123 (51%, 57%) and differed for 788/8 (53%, 60%). The molecular weight of DNA phages calculated from the sum of fragments, obtained after genome splitting with restriction endonucleases is in agreement with the data of sedimentation analysis. The distribution of phage DNA fragments if electrophoregrams suggests the presence of numerous common restriction sites in the phages' genomes.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011549 Pseudomonas A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants. Chryseomonas,Pseudomona,Flavimonas
D002627 Chemistry, Physical The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes. Physical Chemistry,Chemistries, Physical,Physical Chemistries
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D001435 Bacteriophages Viruses whose hosts are bacterial cells. Phages,Bacteriophage,Phage
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D055598 Chemical Phenomena The composition, structure, conformation, and properties of atoms and molecules, and their reaction and interaction processes. Chemical Concepts,Chemical Processes,Physical Chemistry Concepts,Physical Chemistry Processes,Physicochemical Concepts,Physicochemical Phenomena,Physicochemical Processes,Chemical Phenomenon,Chemical Process,Physical Chemistry Phenomena,Physical Chemistry Process,Physicochemical Phenomenon,Physicochemical Process,Chemical Concept,Chemistry Process, Physical,Chemistry Processes, Physical,Concept, Chemical,Concept, Physical Chemistry,Concept, Physicochemical,Concepts, Chemical,Concepts, Physical Chemistry,Concepts, Physicochemical,Phenomena, Chemical,Phenomena, Physical Chemistry,Phenomena, Physicochemical,Phenomenon, Chemical,Phenomenon, Physicochemical,Physical Chemistry Concept,Physicochemical Concept,Process, Chemical,Process, Physical Chemistry,Process, Physicochemical,Processes, Chemical,Processes, Physical Chemistry,Processes, Physicochemical

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