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A DNA polymerase activity induced by human cytomegalovirus (HCMV) was separated from host cell DNA polymerase and purified by phosphocellulose and DNA-cellulose column chromatography. The DNA polymerase activity was strongly inhibited by phosphonoacetic acid, aphidicolin, araATP, and N-ethylmaleimide, but it was resistant to 2',3'-dideoxyTTP. The sensitivity of HCMV-induced DNA polymerase to these reagents resembles that of host cell DNA polymerase alpha. However, HCMV-induced DNA polymerase activity was stimulated several fold by 100 mM ammonium sulfate, by which DNA polymerase alpha activity was strongly inhibited. Furthermore, it was found that a 3'-to-5' exonuclease activity was tightly associated with the HCMV-induced DNA polymerase. The exonuclease was also stimulated by ammonium sulfate, was inhibited by phosphoacetic acid, and it preferred single-stranded DNA as a substrate. The results suggest that the 3'-to-5' exonuclease may play a role in proofreading in the polymerization process as an integral part of the HCMV-induced DNA polymerase.
Y Nishiyama, and
K Maeno, and
S Yoshida
December 1990,
Nucleic acids research,
Y Nishiyama, and
K Maeno, and
S Yoshida
May 1991,
Virology,
Y Nishiyama, and
K Maeno, and
S Yoshida
November 1991,
Biochemistry,
Y Nishiyama, and
K Maeno, and
S Yoshida
January 1992,
Chromosoma,
Y Nishiyama, and
K Maeno, and
S Yoshida
May 1986,
Nucleic acids research,
Y Nishiyama, and
K Maeno, and
S Yoshida
April 1988,
Biochemistry,
Y Nishiyama, and
K Maeno, and
S Yoshida
February 1980,
Nucleic acids research,
Y Nishiyama, and
K Maeno, and
S Yoshida
September 1984,
Biochemical and biophysical research communications,
Y Nishiyama, and
K Maeno, and
S Yoshida
January 1983,
Doklady Akademii nauk SSSR,
Y Nishiyama, and
K Maeno, and
S Yoshida
June 1976,
Biochemistry,
