BIOMAT Database Logo BIOMAT Database Logo

In vivo and in vitro binding of epichlorohydrin to nucleic acids. 1984

M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini

Epichlorohydrin (EC) binds to macromolecules of biological relevance in vivo: DNA is less labelled than RNA and proteins, rat organs interact more than mouse organs, stomach is the most labelled organ with liver, kidney and lung involved in decreasing order. Based on the Covalent Binding Index (CBI), EC is a weak-moderate oncogen, just as other chlorinated hydrocarbons such as 1,2-dichloroethane and carbon tetrachloride. An interaction of EC with nucleic acids (DNA and polyribonucleotides) occurs also in vitro. It is mediated either by chemical reactivity per se of the molecule (near-UV (NUV) irradiation does not photoactivate EC) and by enzymatic (microsomal and/or cytosolic) fractions, whose relative effectiveness is variable in relation to the organ tested. The best substrates for interaction are poly(G) and poly(A) when using microsomal and cytosolic fractions, respectively, whereas the labelling of double-stranded DNA is always lower. On the whole, the picture of enzyme (microsome + cytosol)-mediated in vitro interaction is similar to the pattern of in vivo binding, with the exception of rat stomach enzymes which are inactive in vitro.

UI MeSH Term Description Entries
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008862 Microsomes, Liver Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough. Liver Microsomes,Liver Microsome,Microsome, Liver
D009696 Nucleic Acids High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages. Nucleic Acid,Acid, Nucleic,Acids, Nucleic
D011131 Polyribonucleotides A group of 13 or more ribonucleotides in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D002273 Carcinogens Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included. Carcinogen,Oncogen,Oncogens,Tumor Initiator,Tumor Initiators,Tumor Promoter,Tumor Promoters,Initiator, Tumor,Initiators, Tumor,Promoter, Tumor,Promoters, Tumor
D002728 Chlorohydrins Any of the compounds derived from a group of glycols or polyhydroxy alcohols by chlorine substitution for part of the hydroxyl groups. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Chlorhydrins
D003600 Cytosol Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components. Cytosols

Related Publications

M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
In vivo and in vitro binding of ethionine with nucleic acids.
December 1974, Gan,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
IN VITRO BINDING OF NUCLEIC ACIDS TO TISSUE SECTIONS AFTER REMOVAL OF TISSUE NUCLEIC ACIDS.
August 1964, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
Studies on the binding of 5,5-diphenyl-hydantoin to nucleic acids in vitro and to rat brain subcellular fractions in vitro and in vivo.
February 1976, The Journal of pharmacology and experimental therapeutics,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
Anion binding to nucleic acids.
March 2004, Structure (London, England : 1993),
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
Significance of metabolic activation and binding to nucleic acids of aminostilbene derivatives in vivo.
December 1981, National Cancer Institute monograph,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
BINDING OF MN2+ TO NUCLEIC ACIDS.
January 1965, The Journal of chemical physics,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
In vivo and in vitro binding of carbon tetrachloride with nucleic acids and proteins in rat and mouse liver.
March 1973, International journal of cancer,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
In vitro and in vivo reactions of nucleic acids with reducing sugars.
May 1990, Mutation research,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
Inosine 5'-monophosphate dehydrogenase binds nucleic acids in vitro and in vivo.
April 2004, The Biochemical journal,
M Mazzullo, and A Colacci, and S Grilli, and G Prodi, and G Arfellini
Irreversible binding of acrylonitrile to nucleic acids.
January 1983, Xenobiotica; the fate of foreign compounds in biological systems,
Copied contents to your clipboard!