Biomaterial Database

Rapid serotyping of infectious bronchitis virus isolates with the hemagglutination-inhibition test. 1984

D J King, and S R Hopkins

The hemagglutination-inhibition (HI) test was evaluated as a method of typing recent suspect infectious bronchitis virus (IBV) isolates. Hemagglutination (HA) antigen was prepared from each isolate by phospholipase C treatment of virus concentrated from allanto-amniotic fluids of infected chicken embryos. An HI test was run with the HA antigen of each isolate against a battery of 17 antisera that had been prepared against different IBV strains classified by virus neutralization (VN). The HI test identified Ark 99 and Holland isolates that were similar to strains previously classified by VN. Two isolates included in this study were not inhibited by any of the reference antisera and therefore appeared to be antigenically different. The isolates were also evaluated by VN, and the results of the VN and HI methods agreed. Therefore the results suggest that the HI test can be useful for making a rapid, presumptive identification of new IBV isolates.

UI	MeSH Term	Description	Entries
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003332	Coronaviridae	Spherical RNA viruses, in the order NIDOVIRALES, infecting a wide range of animals including humans. Transmission is by fecal-oral and respiratory routes. Mechanical transmission is also common. There are two genera: CORONAVIRUS and TOROVIRUS.	Bafinivirus,Coronavirinae,Torovirinae,White bream virus,Bafiniviruses,White bream viruses
D006385	Hemagglutination Inhibition Tests	Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.	Hemagglutination Inhibition Test,Inhibition Test, Hemagglutination,Inhibition Tests, Hemagglutination,Test, Hemagglutination Inhibition,Tests, Hemagglutination Inhibition
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D001351	Infectious bronchitis virus	A species of CORONAVIRUS causing infections in chickens and possibly pheasants. Chicks up to four weeks old are the most severely affected.	Avian Infectious Bronchitis Virus,Infectious Bronchitis Virus, Avian,Infectious bronchitis viruses,bronchitis viruses, Infectious
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings