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We report the identification, sequence, and expression in Escherichia coli of the immunodominant epitope of the circumsporozoite (CS) gene of Plasmodium cynomolgi (Gombak), a simian malaria parasite. This epitope is encoded by a DNA sequence that is tandemly repeated 10 times in the cDNA clone. Subclones that contain and express only repeats and in variable number have been constructed. We show that the binding of a specific anti-CS protein monoclonal antibody correlates positively with the number of repeats in each subclone. The CS gene of another strain of P. cynomolgi (NIH) encodes an immunodominant epitope that is immunologically distinct from that of the Gombak strain. We present evidence that these two CS genes share extensive overall homology, although the nucleotide sequences that encode the epitopes appear to be unrelated.
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
April 1986,
Journal of molecular biology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
June 1987,
Experimental parasitology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
January 1985,
Molecular and biochemical parasitology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
February 1989,
The American journal of tropical medicine and hygiene,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
April 1987,
Molecular and biochemical parasitology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
November 1992,
Experimental parasitology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
May 1986,
The American journal of tropical medicine and hygiene,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
December 1997,
Molecular and biochemical parasitology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
August 2001,
Journal of clinical microbiology,
V Enea, and
D Arnot, and
E C Schmidt, and
A Cochrane, and
R Gwadz, and
R S Nussenzweig
November 1997,
Molecular and biochemical parasitology,
