Clostridium pasteurianum is shown to possess constitutive PEP-dependent phosphotransferase systems transporting glucose, fructose, sucrose, sorbitol, and mannitol. With the exception of those for mannitol and sorbitol, each system is specific for a single substrate. At the end of growth, sporulation occurred and there was a coordinate increase in the activity of all the phosphotransferase systems. This increase occurred independently of the nature of the growth substrate.