Fluorescein diacetate staining of Anaplasma marginale as a possible measure of viability. 1978

E E Erp, and M Ristic, and C A Carson

Fluorescein diacetate viability-staining technique was applied to Anaplasma marginale. Marginal bodies took up the stain and became fluorescent. When infected red blood cells were incubated at 55 C, the number of fluorescent Anaplasma decreased at a rate similar to the known loss of viability.

UI MeSH Term Description Entries
D007962 Leukocytes White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES). Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D005452 Fluoresceins A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays. Tetraiodofluorescein
D000709 Anaplasma A genus of gram-negative bacteria whose organisms are obligate parasites of vertebrates. Species are transmitted by arthropod vectors with the host range limited to ruminants. Anaplasma marginale is the most pathogenic species and is the causative agent of severe bovine anaplasmosis.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

E E Erp, and M Ristic, and C A Carson
April 1973, American journal of veterinary research,
E E Erp, and M Ristic, and C A Carson
May 2001, Current microbiology,
E E Erp, and M Ristic, and C A Carson
January 1991, Indian journal of leprosy,
E E Erp, and M Ristic, and C A Carson
October 1957, American journal of veterinary research,
E E Erp, and M Ristic, and C A Carson
January 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
E E Erp, and M Ristic, and C A Carson
October 1984, American journal of veterinary research,
E E Erp, and M Ristic, and C A Carson
January 1993, Biotechnic & histochemistry : official publication of the Biological Stain Commission,
E E Erp, and M Ristic, and C A Carson
December 2016, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
E E Erp, and M Ristic, and C A Carson
May 2017, Journal of visualized experiments : JoVE,
Copied contents to your clipboard!