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To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes, we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition, the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.
S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
December 2011,
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S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
June 1984,
International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association,
S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
September 2012,
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease,
S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
October 1983,
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S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
October 2013,
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S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
February 1978,
American journal of veterinary research,
S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
January 1978,
Animal blood groups and biochemical genetics,
S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
October 1999,
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S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
January 1985,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
S L Nyberg, and
R A Shatford, and
W D Payne, and
W S Hu, and
F B Cerra
February 1990,
Acta ophthalmologica,
