Photodynamic inactivation of pseudorabies virus with methylene blue dye, light, and electricity. 1983

J A Badylak, and G Scherba, and D P Gustafson

Pseudorabies virus was photoinactivated with a combination of methylene blue dye, light, and electricity. Viral suspensions were mixed with variable amounts of methylene blue dye and then were placed in a current source apparatus. Total inactivation of pseudorabies virus (1.7 X 10(6) 50% tissue culture infective doses per ml) was achieved with constant mixing, a methylene blue dye concentration of 10(-4) M, and an electrical current of 12 microA for 12 min.

UI MeSH Term Description Entries
D008027 Light That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range. Light, Visible,Photoradiation,Radiation, Visible,Visible Radiation,Photoradiations,Radiations, Visible,Visible Light,Visible Radiations
D008751 Methylene Blue A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN. Methylthionine Chloride,Swiss Blue,Basic Blue 9,Chromosmon,Methylene Blue N,Methylthioninium Chloride,Urolene Blue,Blue 9, Basic,Blue N, Methylene,Blue, Methylene,Blue, Swiss,Blue, Urolene
D011558 Herpesvirus 1, Suid A species of VARICELLOVIRUS producing a respiratory infection (PSEUDORABIES) in swine, its natural host. It also produces an usually fatal ENCEPHALOMYELITIS in cattle, sheep, dogs, cats, foxes, and mink. Aujeszky's Disease Virus,Swine Herpesvirus 1,Aujeszky Disease Virus,Herpesvirus 1 (alpha), Suid,Herpesvirus Suis,Pseudorabies Virus,Suid Herpesvirus 1,Aujeszkys Disease Virus,Herpesvirus 1, Swine,Pseudorabies Viruses,Virus, Pseudorabies,Viruses, Pseudorabies
D004560 Electricity The physical effects involving the presence of electric charges at rest and in motion.

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