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A kinetic assay system which provides reliable measurements of Na-K-ATPase activity on 0.2 to 0.5-mm segments of renal proximal convoluted tubules isolated from collagenase-digested renal cortical slices is described. The use of collagenase digestion provides higher values for Na-K-ATPase, possibly by making the enzyme more accessible to the reaction system. The advantages of a kinetic vs an endpoint assay include the ability to use the same tubule as its own reference for the determination of total, ouabain-sensitive, and ouabain-insensitive ATPase activity. In addition, it allows dose-response studies on the effect of inhibitors on ATPase activity in the same tubule segment.
G J Schwartz, and
J T Lin, and
R Kinne
January 1995,
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G J Schwartz, and
J T Lin, and
R Kinne
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G J Schwartz, and
J T Lin, and
R Kinne
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G J Schwartz, and
J T Lin, and
R Kinne
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G J Schwartz, and
J T Lin, and
R Kinne
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G J Schwartz, and
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G J Schwartz, and
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G J Schwartz, and
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G J Schwartz, and
J T Lin, and
R Kinne
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G J Schwartz, and
J T Lin, and
R Kinne
February 2000,
Archives of insect biochemistry and physiology,
