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A 32P-postlabeling method for carcinogen-DNA adduct analysis recently developed in our laboratory was applied to skin DNA from mice treated topically with polycyclic aromatic hydrocarbons (PAHs). After application of 4 doses of 1.2 mumol each of benzo[alpha]pyrene (BP), 3-methylcholanthrene (MC) and 7,12-dimethylbenz[alpha]anthracene (DMBA), respectively, total covalent adduct binding in mouse skin DNA initially amounted to 1 adduct in 6.0 X 10(4) - 1.3 X 10(5) nucleotides. Four weeks after treatment, these levels had declined to 1 adduct in 1.4 X 10(6) - 2.7 X 10(6) nucleotides. Substantial removal of DNA adducts occurred during the first 2 weeks after carcinogen application while adducts remaining thereafter underwent little or no repair between 2 and 4 weeks after treatment. These results raise the possibility that the persistent adducts occupy specific genomic sites in quiescent cells where they may not be amenable to repair because of localized conformational alterations of DNA or shielding by associated proteins.
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
March 1993,
Mutagenesis,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
March 1993,
Environmental health perspectives,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
October 1993,
Cancer letters,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
January 1982,
Carcinogenesis,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
January 1990,
Basic life sciences,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
January 2020,
Methods in molecular biology (Clifton, N.J.),
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
January 2005,
Methods in molecular biology (Clifton, N.J.),
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
October 1991,
Journal of toxicology and environmental health,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
August 1988,
Carcinogenesis,
E Randerath, and
H P Agrawal, and
M V Reddy, and
K Randerath
December 1987,
Cancer research,
