Biomaterial Database

Immunoreactive angiotensin I converting enzyme in plasma from normal subjects. Comparison with enzyme activity. 1983

T Yasui, and F Alhenc-Gelas, and S Daufresne

Angiotensin I converting enzyme levels were determined in 18 healthy males using either a direct radioimmunoassay or the enzymatic assay described by Cushman and Cheung (Biochem Pharmac 1971, 20: 1637-48). Mean levels in recumbent position were 2.47 +/- 0.68 pmol ml-1 by radioimmunoassay and 28.1 +/- 6.7 mU ml-1 by enzymatic assay. An excellent correlation (r = 0.86, P less than 0.001) was found between the results obtained with both methods of measurement. The ratio of enzyme activity to immunoreactive angiotensin I converting enzyme levels in plasma was 75.3 +/- 9.5 U mg-1, a value identical to that obtained for the purified enzyme (84 U mg-1). Plasma angiotensin I converting enzyme is immunologically identical to that from the kidney and lung. Comparison of plasma angiotensin I converting enzyme levels in supine and recumbent subjects revealed no effect of posture.

UI	MeSH Term	Description	Entries
D007703	Peptidyl-Dipeptidase A	A peptidyl-dipeptidase that catalyzes the release of a C-terminal dipeptide, oligopeptide-\|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of ANGIOTENSIN I to ANGIOTENSIN II, with increase in vasoconstrictor activity, but no action on angiotensin II. It is also able to inactivate BRADYKININ, a potent vasodilator; and has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. (From https://www.uniprot.org April 15, 2020).	ACE1 Angiotensin-Converting Enzyme 1,ACE1 Protein,Angiotensin Converting Enzyme,Angiotensin Converting Enzyme 1,Antigens, CD143,CD143 Antigens,Dipeptidyl Carboxypeptidase I,Kininase II,Peptidase P,Angiotensin I-Converting Enzyme,Carboxycathepsin,Dipeptidyl Peptidase A,Kininase A,ACE1 Angiotensin Converting Enzyme 1,Angiotensin I Converting Enzyme,Carboxypeptidase I, Dipeptidyl,Peptidyl Dipeptidase A
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D011187	Posture	The position or physical attitude of the body.	Postures
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D004796	Clinical Enzyme Tests	Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.	Enzyme Tests, Clinical,Clinical Enzyme Test,Enzyme Test, Clinical,Test, Clinical Enzyme,Tests, Clinical Enzyme
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults