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The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybrid-selected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pIi-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is know that approximately 20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side.
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
August 1985,
Behring Institute Mitteilungen,
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
February 1991,
Journal of immunology (Baltimore, Md. : 1950),
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
July 1983,
Journal of immunology (Baltimore, Md. : 1950),
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
March 1987,
Journal of immunology (Baltimore, Md. : 1950),
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
January 1990,
Proceedings of the National Academy of Sciences of the United States of America,
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
April 1990,
The Journal of biological chemistry,
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
December 1983,
Proceedings of the National Academy of Sciences of the United States of America,
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
May 1989,
Nucleic acids research,
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
March 1987,
Proceedings of the National Academy of Sciences of the United States of America,
P A Singer, and
W Lauer, and
Z Dembić, and
W E Mayer, and
J Lipp, and
N Koch, and
G Hämmerling, and
J Klein, and
B Dobberstein
November 1987,
The Journal of biological chemistry,
