BIOMAT Database Logo BIOMAT Database Logo

[Permeability of bilayer phospholipid membranes to superoxide oxygen radicals]. 1984

R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin

Lecithin monolayer liposomes (1000 A in diameter) loaded with cytochrome c were placed into the external solution, in which O2 superoxide radicals were regenerated by the xanthine-xanthine oxidase system. The penetration of superoxide radicals across the liposomal membranes was followed by cytochrome c reduction in the interval volume of the liposomes. The effects of lipid membrane modifiers and temperature on this process were investigated. The results obtained were used for calculation of the permeability coefficients of bilayer lipid membranes for O(2) (P'O(2) = (7.6 +/- 0.3) . 10(-8) cm . s-1) or HO . 2(P'HO(2) = 4.9 x 10(-4) cm . s-1). The effect of the transmembrane electric potential (concentration gradient of H+, valinomycin) on the permeability of liposomal membranes for the superoxide radical was studied. The superoxide radical was down to penetrate across the bilayer lipid membranes in an unloaded state. Using an intramolecular cholesterol-amphotericin B-complex, the superoxide radicals were shown to penetrate across the bilayer lipid membranes, predominantly via the anionic channels.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008051 Lipid Bilayers Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes. Bilayers, Lipid,Bilayer, Lipid,Lipid Bilayer
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D008564 Membrane Potentials The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization). Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D010539 Permeability Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions. Permeabilities
D010743 Phospholipids Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system. Phosphatides,Phospholipid
D003574 Cytochrome c Group A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539) Cytochromes Type c,Group, Cytochrome c,Type c, Cytochromes
D000666 Amphotericin B Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela. Amphocil,Amphotericin,Amphotericin B Cholesterol Dispersion,Amphotericin B Colloidal Dispersion,Fungizone
D013481 Superoxides Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides. Superoxide Radical,Superoxide,Superoxide Anion
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
[Isotope permeability of bilayer phospholipid membranes].
February 1980, Tsitologiia,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Proton/hydroxide conductance and permeability through phospholipid bilayer membranes.
September 1987, Proceedings of the National Academy of Sciences of the United States of America,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Superoxide anion permeability of phospholipid membranes and chloroplast thylakoids.
October 1983, Archives of biochemistry and biophysics,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Increasing permeability of phospholipid bilayer membranes to alanine with synthetic alpha-aminophosphonate carriers.
April 2008, Bioorganic & medicinal chemistry letters,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
[Permeability of bilayer lipid membranes to nystatin].
January 1980, Biofizika,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Effects of Amphotericin B on thiourea permeability of phospholipid and cholesterol bilayer membranes.
August 1968, Journal of molecular biology,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Thiourea and potassium permeability of phospholipid bilayer membranes as affected by enniatin B.
April 1968, Nature,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Salicylates and phospholipid bilayer membranes.
May 1973, Nature,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Phospholipid exchange between bilayer membranes.
September 1977, Biochimica et biophysica acta,
R A Gus'kova, and I I Ivanov, and V K Kol'tover, and V V Akhobadze, and A B Rubin
Permeability of phospholipid membranes to inorganic phosphate.
September 1972, Canadian journal of biochemistry,
Copied contents to your clipboard!