Biomaterial Database

[Isoelectric focusing of DNA-methylases from Shigella sonnei 47]. 1983

S V Suchkov, and I I Nikol'skaia, and S S Debov

Fractionation of bacterial methylases was studied by means of isoelectrofocusing technique. The method exhibited high accuracy, efficiency and reproducibility in studies of methylases from Shigella sonnei 47. High column capacity and distinct focusing effect increased the experimental advantages of work with various amounts of protein as compared with ion exchange chromatography. By means of the isoelectrofocusing technique three methylases were detected in the strain S. s o n n e i 47, which were dissimilar in their isoelectric points at pH 6.5, 8.4 and 9.8. These findings indicate the heterogeneous pattern of enzyme methylation in the strain studied. Complete removing of endogenous DNA was the main precondition for correct fractionation of methylases by means of the isoelectrofocusing technique. A procedure is described for purification of S. s o n n e i 47 methylases from traces of endogenous DNA by affinity chromatography on heparin-Sepharose.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008780	Methyltransferases	A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.	Methyltransferase
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D004248	DNA (Cytosine-5-)-Methyltransferases	Enzymes that catalyzes the transfer of a methyl group from S-ADENOSYLMETHIONINE to the 5-position of CYTOSINE residues in DNA.	DNA (Cytosine-5-)-Methyltransferase,DNA Cytosine-5-Methylase,DNA (Cytosine 5) Methyltransferase,Cytosine-5-Methylase, DNA,DNA Cytosine 5 Methylase
D012764	Shigella sonnei	A lactose-fermenting bacterium causing dysentery.	Bacterium sonnei