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Shigella sonnei 47 cells were found to contain DNA-methylase SsoII which is a modifying component of the system of host specificity of SsoII. The recognition sequence (RS) of methylase SsoII is represented by a five-member palyndromic structure--5'...CCNGG...3'--with a degenerated central nucleotide. Modification of SsoII affords protection of acceptor DNA not only from SsoII type restriction, but also from other restrictases, e. g., Eco RII having an analogous RS but with a less degenerated central nucleotide pair. A simple and rapid procedure for isolation and purification of DNA-methylase ScoII, which employs hydrophobic chromatography on phenyl-Sepharose, has been developed. The enzyme preparation does not contain trace amounts of specific and nonspecific endonucleases and keeps stable on storage in 30% glycerol over a period of one year.
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
December 1984,
Biochemistry international,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
October 1985,
Biokhimiia (Moscow, Russia),
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
January 1983,
Voprosy meditsinskoi khimii,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
November 1985,
Biokhimiia (Moscow, Russia),
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
January 1985,
Voprosy meditsinskoi khimii,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
March 1985,
Biochemistry international,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
July 1987,
Biochemistry international,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
April 1985,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
October 1986,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
N G Lopatina, and
E N Lopareva, and
A M Posypanova, and
I I Nikol'skaia, and
S S Debov
January 1979,
Biochimica et biophysica acta,
