A new selective medium for the isolation of Campylobacter jejuni from human faeces. 1983

H Goossens, and M De Boeck, and J P Butzler

A new selective medium, Butzler's medium Virion, for the isolation of Campylobacter jejuni from human faeces is described. This medium contains the following antibiotics per liter: cefoperazone 15 mg, rifampicin 10 mg, colistin 10,000 IU, and amphotericin B 2 mg. At 42 degrees C there was no difference in the isolation rate on Butzler's medium Virion and Butzler's medium Oxoid, but the competing faecal flora was best suppressed by the new medium which allows easier reading of plates and better recognition of campylobacter colonies. Also, the isolation rate and growth of faecal flora were comparable when this new selective medium was incubated at both 37 degrees C and 42 degrees C in a candle jar or a special incubator. These results suggest that Butzler's medium Virion is a very reliable medium for the isolation of campylobacter. In comparison with other selective media it has important advantages for investigators who have little experience in isolating this enteropathogen, or for developing countries where special 42 degrees C incubators are often not available.

UI MeSH Term Description Entries
D002167 Campylobacter A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

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