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We have developed microvolume EMIT procedures for theophylline, phenobarbital, phenytoin, carbamazepine, primidone, ethosuximide, and gentamicin using a centrifugal analyzer (CentrifiChem and Pipettor 1000) to reduce the manufacturer's recommended manual reagent consumption by one-sixth. In addition to developing the EMIT procedure, the performance of the analyzer and pipettor were verified. The analyzer and pipettor are capable of producing within-run precision at a 3-microliters sample volume and 210-microliters analyzer cuvette volume equal to or less than 1.5%. The performance of the EMIT procedures on the analyzer yielded spike drug recoveries of 90.8 to 106.1% for drug concentrations throughout the calibration concentration range of each assay. The percent error on standard reference material of the National Bureau of Standards ranged from a +12.0% to a -0.6% for ethosuximide, phenobarbital, phenytoin, and primidone. Patient comparison data yielded slopes from 0.930 to 1.110 for all assays. The other important feature of the adapted EMIT assay is its simplicity for use on a routine basis.
D Studts, and
G T Haven, and
E J Kiser
January 1981,
The Journal of automatic chemistry,
D Studts, and
G T Haven, and
E J Kiser
February 1981,
Clinical biochemistry,
D Studts, and
G T Haven, and
E J Kiser
May 1989,
Clinical chemistry,
D Studts, and
G T Haven, and
E J Kiser
October 1984,
Clinical chemistry,
D Studts, and
G T Haven, and
E J Kiser
March 1983,
Clinical chemistry,
D Studts, and
G T Haven, and
E J Kiser
August 1985,
Clinical chemistry,
D Studts, and
G T Haven, and
E J Kiser
July 1977,
Clinical chemistry,
D Studts, and
G T Haven, and
E J Kiser
May 1983,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
D Studts, and
G T Haven, and
E J Kiser
January 1982,
Clinical chemistry,
D Studts, and
G T Haven, and
E J Kiser
August 1980,
American journal of clinical pathology,
