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The adaptation of the homogeneous enzyme immunoassays (EMIT) for phenytoin, phenobarbital, and primidone to a centrifugal analyzer is described. The sample volume required was 10 microliter, and the assay had the capacity to analyze sera from 28 patients within 180 sec. The assay temperature was 30 C, and absorbance was monitored at 340 nm. Coefficients of variation for within-day precision ranged from 2.1% to 3.7%, and analytic recovery was quantitative. The centrifugal analyzer EMIT assay results correlated well with those obtained using high-pressure liquid and gas-liquid chromatographic technics. A logit-log transformation of the absorbance rate versus concentration data was obtained using a modified Gauss-Newtonian nonlinear least-squares fit analysis. Severe hemolysis and lipemia caused interference.
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
July 1977,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
December 1976,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
August 1977,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
February 1978,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
February 1981,
Clinical biochemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
July 1978,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
August 1977,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
March 1977,
Clinical chemistry,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
October 1984,
Mutation research,
D A Lacher, and
J A Sinn, and
M R Wills, and
J Savory
January 1983,
Therapeutic drug monitoring,
