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We describe a simple procedure for the microassay of testosterone 5 alpha-reductase in homogenates of rat brain. This enzyme converts testosterone to dihydrotestosterone. We have used this assay to characterize the enzymatic activity and to map its distribution. The apparent Km is 4.1 x 10(-6) M and the Vmax is 85.6 pmol/mg protein/h. The pH optimum is broad and extends from pH 6.0 to 8.0. For the brain regions surveyed, testosterone 5 alpha-reductase activity varied over a 10-fold range. The highest activities were observed in homogenates of the midbrain and pons (37-39 pmol/mg protein/h). The lowest were seen in homogenates of the thalamus, caudate nucleus, frontal cortex, hippocampus, hypothalamus, olfactory tubercle, and preoptic area (3-7 pmol/mg protein/h).
N R Krieger, and
R G Scott, and
M E Jurman
October 1994,
Molecular and cellular neurosciences,
N R Krieger, and
R G Scott, and
M E Jurman
December 1990,
The Journal of antibiotics,
N R Krieger, and
R G Scott, and
M E Jurman
December 1989,
Journal of steroid biochemistry,
N R Krieger, and
R G Scott, and
M E Jurman
January 1983,
Problemy endokrinologii,
N R Krieger, and
R G Scott, and
M E Jurman
October 1973,
Endocrinologia japonica,
N R Krieger, and
R G Scott, and
M E Jurman
October 1981,
Acta medica Okayama,
N R Krieger, and
R G Scott, and
M E Jurman
January 1985,
Steroids,
N R Krieger, and
R G Scott, and
M E Jurman
September 1986,
Endocrinology,
N R Krieger, and
R G Scott, and
M E Jurman
March 1982,
Journal of neurochemistry,
N R Krieger, and
R G Scott, and
M E Jurman
July 1984,
Brain research,
