Biomaterial Database

Adherence of Bacteroides fragilis in vivo. 1978

A B Onderdonk, and N E Moon, and D L Kasper, and J G Bartlett

The ability of the encapsulated species Bacteroides fragilis to adhere to rat peritoneal mesothelium was compared to the adherence of unecapsulated strains of Bacteroides (B. distasonis, B. vulgatus, B. thetaiotaomicron, B. ovatus, and B. "other"). Adherence was assayed by attaching plexiglas plates containing 8-mm holes to the peritoneal mesothelium of anesthetized rats. Cell suspension (0.25 ml) was incubated in each well, after which the suspension was aspirated, and a 4-mm punch biopsy was removed. Viable organisms adhering to the biopsy specimen were enumerated by plate count following washing of the biopsy tissue. It was found that B. fragilis adhered significantly better to mesothelial tissue (10(3.00) colony-forming units per biopsy) than unencapsulated species of Bacteroides (10(1.07) colony-forming units per biopsy). This effect was not due to differential oxygen sensitivity of the various inocula. Immunization of rats with capsular polysaccharide did not demonstrate decrease in the adherence of B. fragilis; however, preincubation of the mesothelium with purified capsular polysaccharide resulted in a substantial reduction in adherence. These results indicate that B. fragilis adheres to rat peritoneal mesothelium better than unencapsulated species and suggests that the capsular polysaccharide of B. fragilis plays some role in this increased adherence.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010537	Peritoneum	A membrane of squamous EPITHELIAL CELLS, the mesothelial cells, covered by apical MICROVILLI that allow rapid absorption of fluid and particles in the PERITONEAL CAVITY. The peritoneum is divided into parietal and visceral components. The parietal peritoneum covers the inside of the ABDOMINAL WALL. The visceral peritoneum covers the intraperitoneal organs. The double-layered peritoneum forms the MESENTERY that suspends these organs from the abdominal wall.	Parietal Peritoneum,Peritoneum, Parietal,Peritoneum, Visceral,Visceral Peritoneum,Parametrium,Parametriums
D011135	Polysaccharides, Bacterial	Polysaccharides found in bacteria and in capsules thereof.	Bacterial Polysaccharides
D002473	Cell Wall	The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.	Cell Walls,Wall, Cell,Walls, Cell
D000267	Tissue Adhesions	Pathological processes consisting of the union of the opposing surfaces of a wound.	Adhesions, Tissue,Surgery-Induced Tissue Adhesions,Surgical Adhesions,Adhesion, Surgery-Induced Tissue,Adhesion, Surgical,Adhesion, Tissue,Adhesions, Surgery-Induced Tissue,Adhesions, Surgical,Surgery Induced Tissue Adhesions,Surgery-Induced Tissue Adhesion,Surgical Adhesion,Tissue Adhesion,Tissue Adhesion, Surgery-Induced,Tissue Adhesions, Surgery-Induced
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies
D000937	Antigen-Antibody Reactions	The processes triggered by interactions of ANTIBODIES with their ANTIGENS.	Antigen Antibody Reactions,Antigen-Antibody Reaction,Reaction, Antigen-Antibody,Reactions, Antigen-Antibody
D001439	Bacteroides	A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
D001441	Bacteroides fragilis	Gram-negative bacteria occurring in the lower intestinal tracts of man and other animals. It is the most common species of anaerobic bacteria isolated from human soft tissue infections.