-transparent.png){kind=logo}
Purine nucleoside phosphorylase purified from chicken liver was inactivated by the sulfhydryl reagent, 4,4'-dithiodipyridine. The inactivation was strongly prevented by the substrates, inosine, phosphate and arsenate. Inactivated enzyme could be reactivated by dithiothreitol. The stoichiometry of modification of sulfhydryl groups was 1.2 residues per mol of the enzyme subunit. The enzyme had five sulfhydryl groups per subunit. One of the five sulfhydryl groups was very reactive with 4,4'-dithiodipyridine, three groups were reactive with excess, 4,4'-dithiodipyridine and another one was masked.
Related Publications
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
January 1987,
Comparative biochemistry and physiology. B, Comparative biochemistry,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
April 1971,
Biochimica et biophysica acta,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
June 1971,
The Journal of biological chemistry,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
April 1975,
Biochimica et biophysica acta,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
January 1985,
Comparative biochemistry and physiology. B, Comparative biochemistry,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
January 1978,
Comparative biochemistry and physiology. B, Comparative biochemistry,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
January 1978,
Methods in enzymology,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
January 1991,
Advances in experimental medicine and biology,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
January 2013,
Biomeditsinskaia khimiia,
S Umemura, and
T Nishino, and
K Murakami, and
K Tsushima
June 1979,
Nihon rinsho. Japanese journal of clinical medicine,
