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Extracts of the bacteria Pseudomonas aeruginosa contain a specific inhibitor of lymphocyte transformation. Considerable purification of this inhibitor was possible by a) smashing the bacteria with glass beads, and b) extracting the soluble components with phosphate buffered saline, c) partial purification with ethanol fractionation, d) ion-exchange column chromatography (DEAE-Sephacel) and e) final purification by elution from Ultrogel. The inhibitor was active at a concentration of approximately 10 ng/ml in inhibiting two-way mixed lymphocyte culture of histoincompatible murine splenic lymphocytes in vitro. The inhibitor was also capable of suppressing the proliferation of the murine leukaemic cell line L1210.
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
July 1983,
Infection and immunity,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
November 1981,
Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
November 1973,
Molecular & general genetics : MGG,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
December 1989,
Nucleic acids research,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
August 1990,
Analytical biochemistry,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
January 1977,
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
August 1976,
Antimicrobial agents and chemotherapy,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
June 1987,
Cell biology international reports,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
January 1986,
Microbiology and immunology,
L M Patt, and
D M Barrantes, and
J Taylor-Norton, and
J C Houck
June 1974,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
