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To identify Rickettsia rickettsii antigens of immunological importance, we examined sera from patients with serologically confirmed cases of Rocky Mountain spotted fever by crossed immunoelectrophoresis for antibodies to antigens extracted from the R strain of R. rickettsii with the detergent Triton X-100. Sixteen antigens were identified in the detergent extract by crossed immunoelectrophoresis with a hyperimmune rabbit serum raised against whole rickettsiae. When the rabbit antiserum was placed in the reference gel and patient sera were placed in the intermediate gel, antibodies to one or more antigens were detected in 61 of 71 North Carolina sera, all of 7 Oklahoma sera, and 9 of 10 Montana sera obtained from 1 day to 40 years after onset of Rocky Mountain spotted fever. Antibodies to antigens 1 and 16 were found as early as 1 day after onset of illness, and antibody to 16 was found in 20 of 29 sera obtained within the first 7 days of illness. Antibodies to antigens 2 and 3 generally did not appear until the third week of illness but were found in six of seven serum samples collected 4 to 40 years after onset of Rocky Mountain spotted fever. Antibodies to R. rickettsii antigens 1, 7, 8, and 16 were found in sera from patients with illnesses caused by other etiological agents. Four of the Oklahoma and Montana sera from Rocky Mountain spotted fever patients, but none of the North Carolina sera, had antibodies to antigen 12. Sera containing antibodies against antigens 3 and 14 prevented death of mice challenged with two 50% lethal doses of R. rickettsii.
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
March 1983,
Journal of clinical microbiology,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
February 1979,
Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
September 1983,
Infection and immunity,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
January 1975,
International journal of fertility,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
January 1982,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
December 1976,
Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
February 1984,
The Journal of infectious diseases,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
February 1987,
Scandinavian journal of immunology,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
July 1976,
Thrombosis research,
R L Anacker, and
R N Philip, and
C M Wilfert, and
K T Kleeman, and
L Turner, and
J N MacCormack, and
K E Hechemy
October 1978,
Experimental eye research,
