-transparent.png){kind=logo}
Simple and rapid Bactec methodologies for the determination of neat (unaltered) and heat stable urease activity of mycobacteria are presented. Clinical isolates (63) and stock cultures (32)--consisting of: M. tuberculosis (19), M. bovis (5), M. kansasii (15), M. marinum (4), M. simiae (3), M. scrofulaceum (16), M. gordonae (6), M. szulgai (6), M. flavescens (1), M. gastri (1), M. intracellulare (6), M. fortuitum-chelonei complex (12), and M. smegmatis (1)--were tested for neat urease activity by Bactec radiometry. Mycobacterial isolates (50-100 mg wet weight) were incubated at 35 degrees C for 30 minutes with microCi14C-urea. Urease-positive mycobacteria gave Bactec growth index (GI) values greater than 100 units, whereas urease-negative species gave values less than 10 GI units. Eighty-three isolates possessing neat urease activity were heated at 80 degrees C for 30 minutes followed by incubation at 35 degrees C for 30 minutes with 1 microCi14C-urea. Mycobacterium tuberculosis-bovis complex demonstrated heat-stable urease activity (GI more than 130 units) and could be distinguished from mycobacteria other than tuberculosis (MOTT), which gave GI values equal to or less than 40 units.
J H Gandy, and
E L Pruden, and
F R Cox
July 1998,
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
J H Gandy, and
E L Pruden, and
F R Cox
June 1990,
Journal of clinical microbiology,
J H Gandy, and
E L Pruden, and
F R Cox
August 1991,
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
J H Gandy, and
E L Pruden, and
F R Cox
April 2000,
Journal of clinical microbiology,
J H Gandy, and
E L Pruden, and
F R Cox
December 1984,
Zhonghua jie he he hu xi xi ji bing za zhi = Chinese journal of tuberculosis and respiratory diseases,
J H Gandy, and
E L Pruden, and
F R Cox
April 1978,
The American review of respiratory disease,
J H Gandy, and
E L Pruden, and
F R Cox
January 2007,
Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia,
J H Gandy, and
E L Pruden, and
F R Cox
January 2003,
Annals of clinical and laboratory science,
J H Gandy, and
E L Pruden, and
F R Cox
October 1982,
The Journal of antimicrobial chemotherapy,
J H Gandy, and
E L Pruden, and
F R Cox
October 1984,
The American review of respiratory disease,
