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We describe a novel separation procedure for immunoradiometric assays involving monoclonal antibodies in which both radiolabeled and capture antibodies are used in solution, the capture antibody being labeled with fluorescein isothiocyanate (FITC). Separation is achieved by incubation with anti-FITC antibodies on magnetic particles. This technique enhances reaction kinetics relative to those of assays in which a solid-phase capture antibody is used, thus allowing faster reaction times and more economic use of the monoclonal antibodies. The use of anti-FITC magnetic solid phase produces an assay having a highly specific separation method, minimal nonspecific binding, and high sensitivity. The method is illustrated by application to assays for thyrotropin and human choriogonadotropin.
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
May 1986,
Annals of internal medicine,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
August 1985,
Clinical chemistry,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
July 1984,
Clinical chemistry,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
July 1987,
Clinical chemistry,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
January 1987,
Clinical chemistry,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
March 1986,
Kaku igaku. The Japanese journal of nuclear medicine,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
December 1985,
The Journal of clinical endocrinology and metabolism,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
June 1987,
Clinical chemistry,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
August 1984,
Clinical chemistry,
S J Rattle, and
D R Purnell, and
P I Williams, and
K Siddle, and
G C Forrest
January 1992,
Clinical chemistry,
